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Updated: Jan 16, 2026
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Quantitative absorptive micro-sampling for decentralized monitoring of insulin-like growth factor 1
Xiaogang Li1, Zeqing Zhao2, Shi Chen3
1Biobank Facility, National Infrastructures for Translational Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Introduction:
This study establishes a novel quantitative acoustic mass spectrometry (QAMS) methodology for insulin-like growth factor 1 (IGF-1) detection.
Methods:
Chromatographic separation utilized a Peptide C18 column (1.8 μm, 50 mm) with 0.1% formic acid/acetonitrile gradient elution, coupled to tandem mass spectrometry operated in scheduled multiple reaction monitoring (sMRM) mode.
Results:
The method demonstrated a lower limit of quantification (LOQ) of 10 ng/mL with linear dynamic range spanning 10-500 ng/mL. Comparative analysis of 74 paired plasma specimens revealed strong inter-matrix correlation with quantifiable bias.
Discussion:
These advancements position QAMS- as a robust tool for decentralized IGF-1 monitoring, particularly valuable in pediatric growth disorder studies and resource-limited settings. Longitudinal stability validation and isoform differentiation remain focal points for future optimization.
