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PlOBP1/PlDAM-PlSOC1 Module Regulates Bud Dormancy Transition in Response to Low Temperature.

Xiaobin Wang1,2, Xiaoxuan Chen1, Kaijing Zhang1

  • 1Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.

Plant, Cell & Environment
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

Low temperatures trigger bud dormancy transition (BDT) in plants. Researchers identified PlSOC1 as a key promoter of BDT and discovered PlOBP1 as a negative regulator, revealing new insights into plant dormancy.

Keywords:
DOFGAMADS‐boxPlOBP1bud dormancy transitionchilling requirementherbaceous peonylow temperature

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Bud dormancy transition (BDT) is crucial for plant survival, regulated by environmental cues like low temperatures.
  • The precise molecular mechanisms governing BDT remain largely unelucidated.

Purpose of the Study:

  • To identify key regulators of bud dormancy transition (BDT) in herbaceous peony (Paeonia lactiflora).
  • To elucidate the molecular mechanisms underlying low-temperature-mediated BDT and the role of gibberellic acid (GA).

Main Methods:

  • Identification of low-temperature-responsive genes using natural and controlled low temperatures.
  • Analysis of transcription factor binding to promoter regions.
  • Gibberellic acid (GA) treatment experiments.
  • Protein-protein interaction studies.

Main Results:

  • A MADS-box gene, SUPPRESSOR OF OVEREXPRESSION OF CO1 (PlSOC1), was identified as a promoter of BDT and low chilling requirement.
  • A DOF transcription factor, OBF BINDING PROTEIN 1 (PlOBP1), was found to negatively regulate BDT by binding to the PlSOC1 promoter.
  • PlOBP1 interacts with PlDAM to enhance PlSOC1 transinhibition.
  • Exogenous GA treatment mimicked chilling by inhibiting PlOBP1 and inducing PlSOC1, promoting BDT.

Conclusions:

  • PlSOC1 and PlOBP1 are key regulators of low-temperature-induced bud dormancy transition (BDT).
  • GA plays a critical role in BDT by modulating the expression of PlOBP1 and PlSOC1.
  • The interaction between DOF (PlOBP1) and MADS-box (PlSOC1, PlDAM) proteins provides mechanistic insights into bud dormancy regulation.