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Related Experiment Video

Updated: Jan 16, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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Liquid biopsy and circulating tumor cell analysis.

Wasiur Rahman Choudhury1, Rama Rao Damerla1, K Devaraja2

  • 1Department of Medical Genetics, Kasturba Medical College and Hospital, Manipal, Manipal Academy of Higher Education, Karnataka, India.

Methods in Cell Biology
|October 1, 2025
PubMed
Summary

This chapter details liquid biopsy methods, covering indications, biofluids, and lab techniques. It explores circulating tumor nucleic acids, cells, extracellular vesicles, and tumor-educated platelets for advanced diagnostics.

Keywords:
Cell free DNACirculating tumor cellsCirculating tumor nucleic acidsCtDNAExtracellular vesiclesLiquid biopsyTumor-educated platelets

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Liquid biopsy offers a minimally invasive approach for cancer detection and monitoring.
  • Analysis of circulating biomarkers in biofluids is revolutionizing personalized medicine.

Purpose of the Study:

  • To provide a comprehensive overview of liquid biopsy techniques.
  • To detail the preanalytical, analytical, and postanalytical aspects of liquid biopsy.
  • To cover key targets including ctNA, CTCs, EVs, and TEPs.

Main Methods:

  • Discussion of indications and suitable biofluids for liquid biopsy.
  • Detailed explanation of laboratory methods for target extraction and enrichment.
  • Emphasis on quality control, storage, and data analysis strategies.

Main Results:

  • Comprehensive description of preanalytical factors impacting liquid biopsy accuracy.
  • Inclusion of methods for analyzing circulating tumor nucleic acids (ctNA).
  • Methods for detecting circulating tumor cells (CTCs), extracellular vesicles (EVs), and tumor-educated platelets (TEPs) are detailed.

Conclusions:

  • Liquid biopsy encompasses a range of sophisticated techniques for biomarker analysis.
  • Standardized preanalytical and analytical methods are crucial for reliable liquid biopsy results.
  • This chapter serves as a foundational resource for understanding and implementing liquid biopsy in clinical and research settings.