Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

475
Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
475

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ru(II)-Catalyzed Hydrodefluorination of Monofluoroalkenes.

The Journal of organic chemistry·2026
Same author

Vaginal microbiota transplantation: from microbial transfer to ecological restoration.

The Lancet. Microbe·2026
Same author

Organic polymer-based monoliths: structural design and integration into microreactors and flow analytical systems.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Effects of deep slow-release fertilizer on melon yield, economic benefits and rhizosphere microorganisms.

Frontiers in plant science·2026
Same author

Cigarette and E-Cigarette Use Among Adolescents Based on a Nationwide Survey - China, 2021.

China CDC weekly·2026
Same author

Noninvasive early detection and grading of pneumoconiosis via plasma proteomics and machine learning: PRSS3 as a potential biomarker.

Clinical proteomics·2026

Related Experiment Video

Updated: Jan 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.4K

Proof-of-concept study of a CD-type microfluidic titration system with an ISFET sensor for microlitre-scale analysis.

Juntao Yang1, Shoji Yamamoto2, Kazuhiro Morioka3

  • 1Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan. yang-juntao@ed.tmu.ac.jp.

Mikrochimica Acta
|October 1, 2025
PubMed
Summary

This study introduces a novel centrifugal microfluidic device with an ion-sensitive field-effect transistor (ISFET) sensor for automated, portable acid-base titration of small liquid samples.

Keywords:
Acid‒base titrationCentrifugal lab-on-a-discISFET sensorMicrofluidicspH monitoring

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.8K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.4K

Related Experiment Videos

Last Updated: Jan 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.4K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.8K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.4K

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Sensor Technology

Background:

  • Conventional titration methods are limited by bulky equipment and large sample requirements, hindering portable and microscale applications.
  • There is a growing need for compact, automated chemical quantification tools for decentralized diagnostics and environmental monitoring.
  • Existing automated titration systems often lack precision or require external pumps and fragile components.

Purpose of the Study:

  • To develop a compact, automated titration system for microlitre-scale samples.
  • To integrate a centrifugal microfluidic device with an ion-sensitive field-effect transistor (ISFET) sensor.
  • To demonstrate precise acid-base titration without external pumps or complex tubing.

Main Methods:

  • A CD-type centrifugal microfluidic device with a 3D-printed chip containing reagent reservoirs was designed.
  • Rotation-driven fluid control was utilized for sequential acid addition.
  • An ion-sensitive field-effect transistor (ISFET) sensor with a Ta₂O₅ membrane and carbon reference electrode was integrated.
  • Automated acid-base titrations were performed on microlitre-scale samples.

Main Results:

  • The ISFET sensor demonstrated high sensitivity (51.3 mV/pH) and linearity (R² = 0.9929).
  • Automated titration of sodium carbonate solution with hydrochloric acid accurately identified equivalence points.
  • Results closely matched those obtained using a commercial pH meter, confirming reliability and reproducibility.
  • The device successfully performed titration on 300 μL samples.

Conclusions:

  • The developed centrifugal microfluidic device with an ISFET sensor enables automated, portable acid-base titration.
  • The system overcomes limitations of conventional titration, offering precise analysis of microlitre-scale samples.
  • This technology holds significant potential for field-deployable chemical analysis, diagnostics, and environmental monitoring.