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Elucidating the Correlation Between Nanozyme-Substrate Binding Constants and Catalytic Efficiency by Affinity
Yuxin Wu1, Tong Xu1, Xin Qian1
1Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331 China.
Analytical Chemistry
|October 2, 2025
Summary
This study reveals a direct link between nanozyme binding affinity and catalytic efficiency using affinity capillary electrophoresis. Understanding nanozyme-substrate interactions offers new insights into their performance.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Nanozymes, nanomaterial-based enzyme mimics, show great potential in various applications.
- Understanding the binding interactions between nanozymes and their substrates is crucial for optimizing their catalytic activity.
- Current methods for characterizing these interactions are limited.
Purpose of the Study:
- To investigate the correlation between nanozyme-substrate binding constants and catalytic efficiency.
- To establish a novel method for studying nanozyme-substrate interactions.
- To provide a deeper understanding of nanozyme catalytic mechanisms.
Main Methods:
- Affinity capillary electrophoresis (ACE), specifically partially filled ACE (PFACE), was employed.
- Two types of oxidase-like nanoceria-based nanozymes (CeO2@PAA and CeO2@PEI) were used as models.
- Binding constants (Kb) with the substrate 3,3',5,5'-tetramethylbenzidine (TMB) were determined.
Main Results:
- Binding constants for CeO2@PAA and CeO2@PEI with TMB were determined as 152.23 and 117.74 L/mol, respectively.
- The binding constant of CeO2@PEI with TMB increased linearly with the concentration of the effector F-.
- A strong linear correlation (r = 0.909) was observed between binding constants and catalytic efficiency (kcat/Km).
Conclusions:
- Affinity capillary electrophoresis provides a new strategy for studying nanozyme-substrate binding.
- The study demonstrates a significant correlation between binding affinity and catalytic efficiency in nanozymes.
- This research offers valuable insights into nanozyme performance by elucidating binding interactions.
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