Deep eutectic solvent extraction of chlorogenic acid from dandelion with ultrasonic-assisted: Process optimization,...

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Biotechnology

Background:

• Dandelion (Taraxacum officinale) is a medicinal plant abundant in chlorogenic acid (CGA).
• Limited research exists on efficient extraction, purification, and bioactivity of CGA from dandelion sources.
• There is a growing demand for natural antioxidants and antibacterial agents in food and pharmaceuticals.

Purpose of the Study:

• To develop an efficient, economic, and optimized method for extracting CGA from dandelion using ultrasonic-assisted deep eutectic solvents (DES).
• To evaluate the antioxidant and antibacterial activities of the purified CGA extract (PECGA).
• To explore the potential applications of dandelion-derived CGA in the food and pharmaceutical industries.

Main Methods:

• Extraction of CGA using ultrasonic-assisted DES (betaine and oxalic acid).
• Optimization of extraction parameters (water content, ultrasonic power, temperature, time, solid-liquid ratio) via Response Surface Methodology (RSM).
• Purification of CGA using macroporous resin chromatography.
• Assessment of antioxidant activity and antibacterial efficacy against Staphylococcus aureus and Escherichia coli.

Main Results:

• The optimal DES extraction conditions yielded 4.27 mg/g of CGA.
• Ultrasound-assisted DES significantly improved extraction efficiency compared to conventional methods.
• Purified CGA extract (PECGA) exhibited notable antioxidant and antibacterial activities.
• PECGA demonstrated significant efficacy against both S. aureus and E. coli.

Conclusions:

• An efficient and economic method for dandelion CGA extraction was successfully developed using ultrasonic-assisted DES.
• The purified CGA from dandelion possesses significant antioxidant and antibacterial properties.
• Dandelion-derived CGA holds promise as a natural ingredient for food preservation and pharmaceutical applications.