Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ILK binding to β1 integrin is indirect and mediated by kindlin-2.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons.

Journal of nanobiotechnology·2026

Same author

Distinct nanoscale membrane organizations of mucins and <i>trans</i>-sialidases in <i>Trypanosoma cruzi</i>.

bioRxiv : the preprint server for biology·2026

Same author

Using DNA Origami to Study Nanoscale Organization of Plasma Membranes.

Nano letters·2026

Same author

Nanoscale Spatial Organization of ARC High- and Low-Order Assemblies at Excitatory Synapses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Post-transcriptional regulatory networks: The dynamic interplay of RNA-binding proteins.

The FEBS journal·2026

Same journal

PCSK5 promotes angiogenesis and cardiac repair after myocardial infarction.

Nature communications·2026

Same journal

PfApiAT2 is a proline transporter essential for the transmission of Plasmodium falciparum by the mosquito vector.

Nature communications·2026

Same journal

Transient distortions of the South Atlantic Anomaly radiation environments driven by electric fields.

Nature communications·2026

Same journal

Structural basis of the regulation by CDK11 kinase of early spliceosome activation and evidence for its proofreading by DHX15 helicase.

Nature communications·2026

Same journal

Structural and mechanistic insights into primer synthesis initiation by DNA primase.

Nature communications·2026

Same journal

Changes in heritability and shared environmentality of educational attainment across twentieth-century Norway.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 20, 2026

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.

Eduard M Unterauer^1,2, Eva-Maria Schentarra^1,2, Isabelle Pachmayr^2,3

¹Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.

Nature Communications

|October 2, 2025

Summary

This summary is machine-generated.

Researchers developed a faster 12-plex imaging method using DNA-PAINT for spatial proteomics. This technique achieves high-resolution mapping of neuronal protein interactions in 3D, overcoming limitations of previous methods.

More Related Videos

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Related Experiment Videos

Last Updated: May 20, 2026

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Area of Science:

Molecular Biology
Microscopy
Proteomics

Background:

Multiplexed super-resolution microscopy allows spatial proteomics at the single-protein level.
Existing methods often require secondary labels, which complicates workflows and reduces throughput.
There is a need for streamlined, high-throughput spatial proteomics techniques.

Purpose of the Study:

To introduce a streamlined approach for rapid and efficient multiplexed super-resolution imaging.
To enable 12-plex imaging with high spatial resolution and throughput.
To map dense neuronal interactomes in 3D.

Main Methods:

Development of a streamlined method combining speed-optimized DNA-PAINT sequences.
Utilization of mirror-image DNA analogs (left-handed DNA) for enhanced imaging.
Validation on synthetic and cellular benchmarks.

Main Results:

Achieved rapid and efficient 12-plex imaging.
Demonstrated mapping of dense neuronal interactomes in three dimensions.
Obtained 15 nm spatial resolution over a 200 × 200 µm² field of view.

Conclusions:

The developed method significantly improves the efficiency and throughput of spatial proteomics.
This technique provides high-resolution 3D mapping of protein interactions in complex biological systems.
The streamlined approach simplifies implementation compared to existing multiplexed microscopy methods.