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Updated: Jan 16, 2026

A Rhodopsin Transport Assay by High-Content Imaging Analysis
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A Rhodopsin Transport Assay by High-Content Imaging Analysis

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Light-Powered Transport of Organic Anions by Microbial Rhodopsins.

Simiao Shen1, Shoichiro Akita1, Joji Wada2

  • 1Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.

The Journal of Physical Chemistry Letters
|October 3, 2025
PubMed
Summary

Microbial rhodopsins, light-activated proteins, can now transport large organic anions, not just small inorganic ions. This discovery expands their known functions in membrane transport.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Microbial rhodopsins are photoactive membrane proteins.
  • They are known for transporting small inorganic ions like H+, Cl-, and Na+.
  • Their structure was thought to limit substrate transport to these ions.

Purpose of the Study:

  • To investigate if microbial rhodopsins can transport organic anions.
  • To characterize the substrate range and transport mechanism of anion-pumping rhodopsins for organic molecules.

Main Methods:

  • Utilized a rhodopsin from cyanobacteria for transport assays.
  • Investigated transport of various organic anions with different volumes and pKa values.
  • Analyzed binding and translocation mechanisms upon photoactivation.

Main Results:

  • Several anion-pumping rhodopsins were found to transport organic anions.
  • A cyanobacterial rhodopsin transported bulky organic anions (up to ~120 Å3), significantly larger than Cl-.
  • Transport was specific to anions with pKa < 2, indicating a requirement for negative charge.

Conclusions:

  • This study presents the first evidence of light-driven transport of organic compounds by naturally occurring proteins.
  • The findings broaden the functional capabilities of microbial rhodopsins.
  • Opens new avenues for light-driven transport systems for organic ions.