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Related Experiment Video

Updated: Jan 16, 2026

Generation of Alginate Microspheres for Biomedical Applications
10:33

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Injectable Cryopreservable Alginate-Gelatin Microbeads for Pro-Angiogenic Cell Therapy.

Francesco Touani Kameni1,2, Sara Badr1,3, Yohann Vigouroux1,3

  • 1Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, QC H2X 0A9, Canada.

ACS Biomaterials Science & Engineering
|October 4, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed oil-free microbeads to improve mesenchymal stromal cell (MSC) therapy for ischemic tissues. These injectable, cryopreservable alginate-gelatin microbeads enhance MSC survival and paracrine function, showing promise for clinical applications.

Keywords:
air assisted coaxial systemalginate-gelatincell therapycryopreservationinjectable microbeadsischemic disease

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Last Updated: Jan 16, 2026

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3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

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Area of Science:

  • Biomaterials Science
  • Cell Therapy Engineering
  • Tissue Engineering

Background:

  • Mesenchymal stromal cell (MSC) therapy faces challenges in cell survival, retention, and paracrine function for ischemic tissues.
  • Developing clinically transferable and storable MSC delivery systems is crucial for therapeutic efficacy.

Purpose of the Study:

  • To develop an oil-free, injectable, and cryopreservable biomaterial microbead system for encapsulating MSCs.
  • To optimize the alginate-gelatin (ALG-Gel) microbead formulation for enhanced MSC viability, function, and stability.

Main Methods:

  • An air-assisted coaxial system was used to create oil-free ALG-Gel microbeads encapsulating MSCs.
  • Microbead characteristics (size, injectability, mechanical properties, stability) were evaluated at varying gelatin concentrations.
  • MSC viability, vascular endothelial growth factor (VEGF) release, and paracrine function were assessed in vitro, pre- and post-cryopreservation.

Main Results:

  • Oil-free ALG-Gel microbeads (400-500 μm) were successfully fabricated with good injectability and mechanical stability.
  • The ALG-Gel3% formulation demonstrated superior MSC survival and wound closure rates compared to ALG alone.
  • Cryopreservation impacted mechanical strength, cell survival, and VEGF release, but cell viability remained >80% post-thaw, with retained pro-angiogenic potential.

Conclusions:

  • Oil-free, injectable, and cryopreservable ALG-Gel microbeads offer a promising platform for enhancing MSC therapy safety and efficacy.
  • Further optimization of cryopreservation protocols and in vivo validation are necessary to confirm therapeutic potential in ischemic models.