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Updated: Jan 16, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Published on: April 23, 2014
Generation and validation of a iPSC line from a healthy female donor using integration-free Sendai virus
Yuanyuan Chen1, Xiaoqin Shi1, Wei Long1
1Department of Ophthalmology, First People's Hospital of Yibin, No. 16 Puhe East Road, Yibin 644000 Sichuan, PR China.
Abstract:
We generated a characterized integration-free iPSC line derived via Sendai virus reprogramming from healthy female donor PBMCs. This method preserves genomic integrity and pluripotent potential. Validated markers include nuclear/membrane-bound OCT4, SOX2, NANOG/SSEA4, TRA-1-60 (qPCR/immunocytochemistry) and tri-lineage differentiation capacity confirmed via immunofluorescence (endodermal FOXA2/SOX17, mesodermal BRACHYURY/NKX2.5, ectodermal PAX6/NESTIN). Normal karyotype (46,XX) confirmed genetic stability. Sendai clearance by P10 and mycoplasma-negative status ensured biosafety. This non-integrative iPSC line provides a robust control resource for disease modeling and regenerative medicine.

