Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

27.5K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
27.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Single Amino Acid Substitution Reprograms ROS Selectivity and Catalytic Function in DyP Peroxidases.

Inorganic chemistry·2026
Same author

Plug-and-(Dis)Play Epitope Engineering on Ring-like Particles: Rational Design of Multivalent Immunoreagents for Diagnostics.

ACS applied bio materials·2026
Same author

Immobilization and electrochemical activation synergistically enhance activity, stability and solvent tolerance of an unspecific peroxygenase.

Bioresource technology·2026
Same author

Myoglobin-Membrane Association Facilitates Oxygen Release via Active-Site Tuning.

Journal of the American Chemical Society·2026
Same author

Passive immunotherapy for Parkinson's disease in 2025: Status and perspective.

Neural regeneration research·2026
Same author

Membrane-mimicking surfaces modulate the heme pocket structure and oxygen affinity in myoglobin: A surface-enhanced resonance Raman study.

Colloids and surfaces. B, Biointerfaces·2026

Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Multivalent Protein Nanorings for Broad and Potent SARS-CoV-2 Neutralization.

Molood Behbahanipour1, Carla Goldin2,3, Cecilia Arahi Prato3,4

  • 1Institut de Biotecnologia i de Biomedicina (IBB) and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra (Barcelona), 08193, Spain.

Advanced Healthcare Materials
|October 6, 2025
PubMed
Summary

A novel nanoscaffold displays engineered binders to neutralize SARS-CoV-2, including Omicron variants. This protein-only system offers a scalable, cost-efficient platform for both antiviral therapy and diagnostics, addressing limitations of antibody-based treatments.

Keywords:
SARS‐CoV‐2minibindersprotein scaffoldtherapeuticviral neutralization

More Related Videos

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.7K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K

Related Experiment Videos

Last Updated: Jan 16, 2026

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K
Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.7K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Virology

Background:

  • Conventional antibody-based therapeutics (ABTs) for SARS-CoV-2 face limitations in scalability and customization.
  • There is a critical need for advanced antiviral platforms to combat ongoing viral threats like SARS-CoV-2.

Purpose of the Study:

  • To develop a modular, protein-only nanoscaffold system for potent SARS-CoV-2 neutralization and diagnostics.
  • To engineer and display de novo-designed minibinders targeting the SARS-CoV-2 Spike protein on a recombinant ring-like particle (RLP) scaffold.

Main Methods:

  • Utilized a viral nucleoprotein (NP) to create recombinant ring-like particles (RLPs).
  • Engineered high-affinity minibinders (LCB1, LCB3) and fused them to RLPs for multivalent display.
  • Assessed neutralization of pseudotyped and authentic SARS-CoV-2 (including Omicron BA.5) and developed an ELISA diagnostic assay.

Main Results:

  • The RLP-minibinder nanoparticles potently inhibited SARS-CoV-2 Spike-ACE2 interactions.
  • Dual-display nanoparticles (RLP-1,3) demonstrated synergistic binding with sub-fM IC50 values, outperforming benchmarks.
  • Achieved a 9 ng/mL detection limit for Spike protein in an ELISA, surpassing commercial assays.

Conclusions:

  • The RLP-minibinder system provides a generalizable, scalable, and cost-efficient platform for integrated antiviral therapy and diagnostics.
  • This plug-and-(dis)play bio-nanomaterial offers a flexible alternative to ABTs for pandemic preparedness.
  • Convergence of AI-guided design and nanoscaffolding enables next-generation multifunctional nanomaterials.