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Updated: Jan 16, 2026
A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
Published on: February 17, 2023
ROS scavenging Mn3O4 nanozyme regulated immune microenvironment and affects intercellular interaction to promote
Zhuoyuan Li1, Ao Zheng2, Chen Liang1
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Abstract:
Diabetes-induced chronic wound healing poses significant clinical and economic challenges. In the pathological context of diabetic wounds, the accumulation of reactive oxygen species (ROS) and inflammatory factors is exacerbated, impeding the transition of macrophages from the M1 to M2 phenotype, thereby leading to prolonged wound healing. Therefore, this study has developed an ultra-small tri-manganese tetroxide nanozyme with dual superoxide dismutase/catalase enzymatic activities, which exhibits excellent ROS scavenging performance. Under oxidative stress conditions, this nanozyme can alleviate mitochondrial damage and promote the transition of macrophages from the M1 to M2 phenotype, thereby mitigating the inhibition of cellular function caused by the inflammatory state through intercellular interactions. Furthermore, the application of this nanozyme in vivo has also contributed to the treatment of skin defects in streptozotocin-induced diabetic mice by alleviating inflammation and scavenging ROS. The dual-enzymatic nanozyme designed and prepared in this study, which scavenges ROS, can regulate the local immune microenvironment and intercellular interactions, providing a new strategy for the clinical treatment of diabetic wound healing.
Insights
This study introduces a novel nanozyme that effectively scavenges reactive oxygen species (ROS), promoting diabetic wound healing by modulating macrophage function and reducing inflammation.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Wound Healing Research
Background:
- Diabetic wound healing is a significant clinical challenge due to excessive reactive oxygen species (ROS) and inflammation.
- Impaired macrophage polarization (M1 to M2 phenotype) prolongs the healing process in diabetic conditions.
Purpose of the Study:
- To develop an ultra-small nanozyme with dual superoxide dismutase/catalase activities for ROS scavenging.
- To investigate the nanozyme's efficacy in alleviating oxidative stress and inflammation in diabetic wound models.
Main Methods:
- Synthesis of an ultra-small tri-manganese tetroxide nanozyme with dual enzymatic activities.
- Evaluation of ROS scavenging capabilities under oxidative stress.
- Assessment of macrophage phenotype transition and intercellular interactions.
- In vivo testing on streptozotocin-induced diabetic mice with skin defects.
Main Results:
- The nanozyme effectively scavenges ROS, mitigating mitochondrial damage.
- It promotes the M1 to M2 macrophage phenotype transition, crucial for healing.
- In vivo studies demonstrated reduced inflammation and improved skin defect treatment in diabetic mice.
Conclusions:
- The developed dual-enzymatic nanozyme offers a promising therapeutic strategy for diabetic wound healing.
- It effectively regulates the immune microenvironment and intercellular interactions by scavenging ROS.
- This provides a novel approach for clinical applications in managing diabetic complications.
