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Updated: Jan 16, 2026

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
Exclusion of the endoplasmic reticulum from the C. elegans meiotic spindle controls spindle size
Alma Aquino1, Francis McNally1
1Molecular and Cellular Biology, University of California, Davis, Davis, California, United States.
Abstract:
The nuclear envelope is composed of sheet-like ER that separates chromatin from tubulin during interphase. During oocyte maturation in C. elegans , a fenestrated envelope of sheet-like ER continues to envelope the meiotic spindle through metaphase I. ER is thus excluded from the nuclear/spindle volume during spindle assembly. To test the importance of this exclusion, we forced ER into the meiotic spindle by coupling kinesin motor domains to the ER. Forcing ER into the spindle interior caused a statistically significant increase in metaphase spindle width. Exclusion of ER from the spindle thus affects spindle geometry.
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