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Updated: Jan 11, 2026

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Published on: February 14, 2016
Y45G5AL.1 is a potential Kinesin-1 Interacting Protein on the Surface of Yolk Granules
Denisa Lazureanu1, Francis McNally1
1Molecular and Cellular Biology, University of California, Davis, Davis, California, United States.
Abstract:
In C. elegans , kinesin/ KCA-1 on the surface of yolk granules is thought to drive outward migration of the meiotic spindle by transporting membranous organelles inward on microtubules with minus ends at the cortex. Here we used BioID to identify Y45G5AL.1 as a potential KCA-1-interacting protein. Y45G5AL.1 co-localized with KCA-1 on 20% of membrane vesicles and a constitutively active kinesin-1 prematurely packed Y45G5AL.1 vesicles into a central ball in diakinesis oocytes. These results indicate that Y45G5AL.1 is on the cytoplasmic surface of endosomes that are direct cargo of kinesin 1.
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