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Ogremorphin inhibits GPR68 mediated MUC5AC expression.
Leif R Neitzel1,2, Hussain F Basrai1,2, Charles C Hong1,2
1Medicine, Michigan State University, East Lansing, Michigan, United States.
Micropublication Biology
|October 6, 2025
Summary
Targeting the GPR68 receptor with Ogremorphin (OGM) can reduce MUC5AC mucus production. This finding offers a potential therapy for lung diseases by improving airway clearance and inhibiting tumor progression.
Area of Science:
- Biochemistry
- Cell Biology
- Pulmonary Medicine
Background:
- Mucus hypersecretion, particularly MUC5AC, worsens airway obstruction in lung diseases like asthma and aids tumor growth.
- The pH-sensing receptor GPR68 is a key regulator of MUC5AC expression.
Purpose of the Study:
- To investigate the role of GPR68 in mediating MUC5AC expression under acidic conditions.
- To evaluate the efficacy of the GPR68 inhibitor Ogremorphin (OGM) in reducing MUC5AC production.
Main Methods:
- Utilized A549 lung cancer cells to study MUC5AC mRNA and protein expression.
- Applied acidic conditions (pH 6.4) and treated cells with OGM.
- Confirmed GPR68's role using shRNA-mediated knockdown.
Main Results:
- Acidification (pH 6.4) significantly increased MUC5AC mRNA and protein levels in a GPR68-dependent manner.
- OGM treatment effectively reduced MUC5AC expression, independent of pH.
- GPR68 knockdown corroborated the inhibitory effect of OGM on MUC5AC production.
Conclusions:
- GPR68 signaling is crucial for MUC5AC hypersecretion.
- GPR68 inhibition via OGM presents a promising therapeutic strategy for managing mucus hypersecretion in lung diseases.
- This approach may improve airway clearance and limit tumor progression in pulmonary conditions.
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