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iTRAQ-Based Proteomic Approach to Study Stress Memory Responses in Plants.

Monika Chouhan1,2, Paramdeep Kumar1, Rajiv Kumar3,4

  • 1Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, HP, India.

Methods in Molecular Biology (Clifton, N.J.)
|October 6, 2025
PubMed
Summary

Plants can remember past stresses, enhancing future responses through protein priming and epigenetic regulation. This study details using Isobaric tags for relative and absolute quantification (iTRAQ) for plant proteomics research.

Keywords:
AcetonitrileMass spectrometryPeptidePlant tissueStress memoryStrong cation exchangeiTRAQ

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Area of Science:

  • Plant Biology
  • Proteomics
  • Molecular Biology

Background:

  • Plants exhibit stress memory, enhancing responses to recurring environmental challenges.
  • Stress memory involves protein confirmation, epigenetic modifications, and hormonal/metabolic signatures.
  • Comparative proteomics is crucial for understanding protein functions under stress.

Purpose of the Study:

  • To provide a detailed protocol for applying Isobaric tags for relative and absolute quantification (iTRAQ) in plant proteomics.
  • To illustrate the utility of iTRAQ for quantifying protein changes in response to plant stress.
  • To discuss the general applications of iTRAQ in plant science.

Main Methods:

  • Detailed protocol for protein isolation and peptide labeling using iTRAQ.
  • Protein fractionation and Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) analysis.
  • Data analysis strategies for iTRAQ-based quantitative proteomics.

Main Results:

  • The described iTRAQ protocol enables robust protein quantification in plant samples.
  • iTRAQ facilitates the identification of proteins involved in plant stress memory.
  • The method allows for comparative analysis of proteomes under different stress conditions.

Conclusions:

  • iTRAQ is a powerful technique for advancing plant proteomics research, particularly in stress response studies.
  • The detailed protocol serves as a valuable resource for researchers investigating plant stress memory.
  • Understanding plant stress memory through proteomics can lead to improved crop resilience.