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Curcuma kwangsiensis-derived nanovesicles enhance periodontal cell functionality through multicellular crosstalk in
Qian Lan1, Xin Xiao1, Xueting Bi1
1Foshan Stomatology Hospital and School of Medicine, Foshan University, Foshan, Guangdong Province, China.
Abstract:
Plant-derived nanovesicles exhibit excellent biocompatibility and multifunctionality, making them promising for tissue regeneration. This study investigates the regenerative potential of Curcuma kwangsiensis-derived nanovesicle (CKNV) in periodontal repair. The morphology, size, surface charge, and bioactive cargo of CKNV were evaluated by transmission electron microscopy, nanoparticle tracking analysis, and spectrophotometry. In vitro experiments were carried out to assess the interaction of CKNV with periodontal ligament stem cells (PDLSCs), macrophages, and vascular endothelial cells (HVECs). The results indicated that CKNV significantly promoted the proliferation and migration of PDLSCs within the concentration range of 10-50 μg/mL in a dose-dependent manner. In addition, CKNV polarized macrophages toward the M2 phenotype. Moreover, CKNV-induced M2 macrophages stimulated angiogenesis in HVECs and facilitated osteogenic differentiation of PDLSCs. Our findings suggest that CKNV modulates periodontal tissue regeneration through multi-pathway synergy: activating periodontal ligament stem cells, polarizing macrophages toward a pro-repair M2 phenotype, promoting angiogenesis, and facilitating osteogenic differentiation. This discovery offers a novel idea for the development of targeted treatment for periodontitis.
