Integrated and turnkey custom-built multiplexed portable platform for on-site electrochemical detection.

Area of Science:

• Electrochemistry
• Biosensing
• Lab-on-Chip (LOC) technology

Background:

• Multiplexed biosensing is crucial for point-of-care diagnostics but hindered by bulky, expensive equipment.
• Electrochemical sensing offers sensitivity and scalability for LOC platforms.
• Conventional potentiostats are not suitable for portable, cost-effective, multiplexed applications.

Purpose of the Study:

• To develop a low-cost, portable potentiostat with multiplexed electrochemical biosensing capabilities and Wi-Fi connectivity.
• To enable real-time detection of multiple analytes for rapid and comprehensive biosensing.
• To provide an accessible solution for clinical and resource-limited settings.

Main Methods:

• Development of a portable potentiostat (μBIOPOT) with a ±1 V potential window and ±1 mA current range.
• Implementation of various voltammetric techniques: Cyclic Voltammetry (CV), Linear Sweep Voltammetry (LSV), Differential Pulse Voltammetry (DPV), Square Wave Voltammetry (SWV), and Normal Pulse Voltammetry (NPV).
• Performance validation using a ferri/ferrocyanide redox probe and detection of lipid biomarkers (cholesterol, triglycerides).

Main Results:

• The μBIOPOT demonstrated performance comparable to conventional potentiostats.
• Successful multiplexed detection of cholesterol and triglycerides was achieved.
• The device offers user-friendly interfaces for real-time data visualization and biomarker quantification.

Conclusions:

• The μBIOPOT is a cost-effective ($36), portable, and scalable solution for multiplexed electrochemical biosensing.
• Its Wi-Fi connectivity and low power consumption enhance its utility in diverse settings.
• This device facilitates the advancement of point-of-care diagnostics and research in resource-limited environments.