Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

468
Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
468
Liver Regeneration01:24

Liver Regeneration

4.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.3K
Whole Body Regeneration01:33

Whole Body Regeneration

4.0K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.0K
Heart Failure VI: Adjunct Therapies01:22

Heart Failure VI: Adjunct Therapies

255
Additional therapies for treating patients with heart failure (HF) may include procedural interventions, supplemental oxygen, the management of sleep disorders, and nutritional therapy.Procedural InterventionsImplantable Cardioverter-Defibrillator: For patients at risk of life-threatening arrhythmias due to severe left ventricular dysfunction, an Implantable Cardioverter-Defibrillator (ICD) can detect and terminate these arrhythmias, preventing sudden cardiac death and improving survival rates.
255

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of Anti-Viral Compounds from <i>Toxicodendron vernicifluum</i> Extract That Inhibit the Coronavirus Replication.

Journal of microbiology and biotechnology·2026
Same author

Zebrafish knock-in lines enabling live visualization of extracellular matrix dynamics during development and regeneration.

Development (Cambridge, England)·2026
Same author

Long-term editing of brain circuits using an engineered electrical synapse.

Nature·2026
Same author

Q-Needle-Assisted Intraductal Injection Enhances Dacryoendoscopic Surgery for Primary Acquired Lacrimal Drainage Obstruction: A Retrospective Study.

Journal of clinical medicine·2026
Same author

Classifying germline and somatic structural variants in tumor-only contexts using GaTSV.

STAR protocols·2026
Same author

MRE11 proximal polyadenylation site-mediated looping impacts transcription and genomic stability.

Molecular cell·2026
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
Same journal

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jan 15, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
10:42

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

9.5K

Ddx61-enriched condensates refine heart regeneration programs.

Mira I Pronobis1,2,3, Sophia DeLuca4, Siyun Lee1

  • 1Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, NC, USA.

Nature Communications
|October 9, 2025
PubMed
Summary
This summary is machine-generated.

RNA helicase Ddx61 forms condensates in heart cells, regulating cardiomyocyte proliferation and tissue regeneration. This discovery offers new insights into heart repair mechanisms and potential therapeutic targets for cardiac injury.

More Related Videos

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
08:13

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Published on: April 10, 2019

6.4K
An Apical Resection Model in the Adult Xenopus tropicalis Heart
05:45

An Apical Resection Model in the Adult Xenopus tropicalis Heart

Published on: November 18, 2022

1.7K

Related Experiment Videos

Last Updated: Jan 15, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
10:42

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

9.5K
Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
08:13

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Published on: April 10, 2019

6.4K
An Apical Resection Model in the Adult Xenopus tropicalis Heart
05:45

An Apical Resection Model in the Adult Xenopus tropicalis Heart

Published on: November 18, 2022

1.7K

Area of Science:

  • Cardiovascular Biology
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Gene regulation in tissue regeneration is primarily studied at the transcriptional level.
  • The role of RNA-binding proteins and their condensates in cardiac regeneration remains underexplored.

Purpose of the Study:

  • To investigate the role of RNA helicase Ddx61 in cardiomyocyte proliferation and heart regeneration.
  • To elucidate the molecular mechanisms by which Ddx61 influences cardiac repair.

Main Methods:

  • Proximity labeling techniques to identify Ddx61 in cardiomyocytes.
  • Analysis of ddx61 mutations in zebrafish and DDX6 knockdown in human cells.
  • Assessment of Ddx61's association with Chordin mRNA during heart regeneration.

Main Results:

  • Ddx61 protein levels and condensate formation increase in dividing cardiomyocytes following injury or mitogen stimulation.
  • ddx61 mutations impair zebrafish heart regeneration, while DDX6 knockdown reduces human cardiomyocyte proliferation.
  • Ddx61 restrains Chordin mRNA expression, a key factor in cardiac regeneration.

Conclusions:

  • Ddx61 plays a critical role in regulating cardiomyocyte proliferation and heart regeneration.
  • Context-dependent mRNA sorting by Ddx61 condensates impacts tissue regenerative capacity.
  • Ddx61 represents a potential therapeutic target for enhancing cardiac repair.