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Highly efficient transgenesis mediated by Tip100 transposon system in medaka
Yoshitaka Tanaka1,2, Takahide Seki3, Atsushi Hoshino4,5
1Ushimado Marine Institute (UMI), Okayama University, Setouchi, Okayama, 701-4303, Japan.
Transgenic Research
|October 9, 2025
Summary
The Tip100 transposon system from plants enables efficient and stable transgenesis in Japanese medaka fish. This method allows for reliable genetic tool expression and the creation of new transgenic medaka strains.
Area of Science:
- Genetics
- Molecular Biology
- Aquaculture
Background:
- Transposon-mediated transgenesis is crucial for creating transgenic organisms.
- The Tol2 transposon is effective in zebrafish but unsuitable for medaka due to endogenous activity.
- Medaka (Oryzias latipes) require a novel transgenesis tool for stable genetic modification.
Purpose of the Study:
- To evaluate the DNA transposon Tip100 from Ipomoea purpurea as a transgenesis tool in Japanese medaka.
- To assess the efficiency of Tip100 in generating stable transgenic medaka lines.
- To compare Tip100 performance with existing transgenesis methods.
Main Methods:
- Co-injection of GFP transgene cassette with Tip100 transposase mRNA in medaka embryos.
- Utilizing truncated and full recognition sequences for the transgene.
- Employing a germline-specific promoter for germline transmission studies.
- Analyzing transgene integration sites and duplication patterns.
Main Results:
- Tip100 significantly increased GFP expression in somatic cells of injected medaka.
- Truncated Tip100 recognition sequences maintained high transgene expression levels.
- Germline transmission rates were significantly higher with Tip100 mRNA (62.7%) compared to controls (21.0%).
- Stable transgenic medaka strains were successfully established with random integration and 8-bp duplications.
Conclusions:
- The Tip100 transposon system is a highly effective tool for transgenesis in Japanese medaka.
- Tip100 facilitates the generation of stable transgenic medaka lines for expressing genetic tools.
- This system holds potential for transgenesis applications in other fish species.
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