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Updated: Jun 12, 2026
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Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)
Published on: October 6, 2022
Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka
Miwa Kuroyanagi1, Satoshi Ansai2, Keigo Okamoto3,4
1Department of Advanced Aquaculture Science, Faculty of Marine Bioscience and Technology, Fukui Prefectural University, Obama, Fukui, Japan.
Development, Growth & Differentiation
|June 10, 2026
Summary
A standardized protocol for assessing Japanese medaka sperm motility using computer-aided sperm motility analysis (CASA) ensures reproducible results. Early measurements post-activation are crucial for accurate data, especially when evaluating cryopreserved sperm.
Area of Science:
- Reproductive Biology
- Aquatic Toxicology
- Environmental Risk Assessment
Background:
- Sperm motility is a key indicator of reproductive health, sensitive to environmental factors.
- Inconsistent measurements hinder accurate assessment of sperm motility.
- Standardization is needed for reliable sperm motility analysis in research.
Purpose of the Study:
- To develop a standardized protocol for assessing Japanese medaka sperm motility.
- To evaluate the impact of cryopreservation on sperm motility.
- To establish optimal timing for post-activation motility measurements.
Main Methods:
- Utilized a sperm motility analysis system (SMAS) with computer-aided sperm motility analysis (CASA).
- Detailed procedures for sperm collection, activation, and quantitative motility assessment.
- Investigated time-dependent changes and cryopreservation effects on motility.
Main Results:
- Demonstrated time-dependent declines in sperm motility and velocity post-activation.
- Cryopreservation accelerated motility decline but did not affect initial motile sperm proportion.
- Early measurements (within 1 minute) are critical for capturing peak motility.
Conclusions:
- The standardized SMAS-based protocol offers a robust framework for medaka sperm motility analysis.
- Early post-activation measurements are essential for consistent and reliable data.
- This protocol supports research in reproductive biology, toxicology, and aquacultural breeding.
