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Updated: Jul 22, 2026

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
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H3NGST: a fully automated, web-based platform for end-to-end ChIP-seq analysis

Hyeon Ho Heo1, Soo-Jong Um2

  • 1Department of Integrative Bioscience and Biotechnology, Sejong University, 209 Neungdong-Ro, Gwangjin-Gu, Seoul, 05006, South Korea.

BMC Bioinformatics
|October 9, 2025
PubMed
Summary

No abstract available in PubMed .

Keywords:
Automated analysisChIP-seqEpigenomicsNext-generation sequencingWeb-based pipeline

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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