Related Experiment Video
Updated: Jan 15, 2026
11:47
Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
2.1K
Breaking through Microbial Defenses─Organic Acid-Based Deep Eutectic Solvents as a Neoteric Strategy in Bacterial
Tomasz Swebocki1, Aleksandra M Kocot2, Karolina Cieminska2
1Institute of Nanotechnology and Materials Engineering, Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, 11/12 G. Narutowicza Street, Gdańsk 80-233, Poland.
ACS Applied Bio Materials
|October 10, 2025
Summary
Organic acid-based deep eutectic solvents (OA-DESs) show potent antimicrobial activity, effectively eradicating biofilms and inhibiting fungal growth. These novel agents offer a promising alternative for combating resistant microbial infections.
Area of Science:
- Green Chemistry
- Microbiology
- Antimicrobial Research
Background:
- Organic acid-based deep eutectic solvents (OA-DESs) have shown efficacy against planktonic bacteria.
- Further investigation is needed to assess their potential against more complex microbial structures and organisms.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of OA-DESs against bacterial biofilms, persister cells, and pathogenic and phytopathogenic fungi.
- To compare OA-DESs with conventional deep eutectic solvents (DESs).
Main Methods:
- Testing OA-DESs against biofilms of methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli.
- Assessing the impact of OA-DESs on bacterial persister cells.
- Evaluating OA-DESs against Candida albicans, Candida auris, Aspergillus fumigatus, Zymoseptoria tritici, and Venturia inaequalis.
- Comparing OA-DESs with conventional DESs.
Main Results:
- OA-DESs effectively eradicated MRSA and E. coli biofilms, causing significant morphological changes and achieving a three-log-unit reduction at low concentrations.
- Most OA-DESs did not promote persister cell formation.
- OA-DESs demonstrated significant inhibition of fungal growth and induced morphological changes in pathogenic fungi.
- OA-DESs showed inhibitory effects on phytopathogenic fungi, though less pronounced than on pathogenic strains.
Conclusions:
- OA-DESs are effective antimicrobial agents against bacterial biofilms and fungi.
- OA-DESs present a promising avenue for developing novel antimicrobial strategies.
- Further research into OA-DESs can lead to innovations in antimicrobial applications.
Related Concept Videos
01:28Biological Methods for Microbial Control
783
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
783
01:27Chemical Agents for Microbial Control
759
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
759
01:18Surface Membrane Barriers
2.6K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.6K
01:29Biofilms
1.1K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.1K
01:28Antimicrobial Effectiveness
904
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
904
01:27Microorganisms in Agriculture and Food industry
1.3K
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
1.3K