Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancing myrcene biosynthesis in yeast through nuclear compartmentalization.

Synthetic and systems biotechnology·2026

Same author

Application of the Ridge Board Technique in Endoscopic Treatment of Cervical Stenosis: Surgical Technique and Preliminary Results.

World neurosurgery·2026

Same author

Prediction of the Compressive Strength of Tailings-Based Cement Material Using Machine Learning Models with Experimental Validation.

Materials (Basel, Switzerland)·2026

Same author

NCOA4-driven ferritinophagy and GSH reprogramming underlie maslinic acid-induced ferroptosis and autophagy in breast cancer.

Translational cancer research·2026

Same author

Structural and mechanistic insights into the VP14460-VP14465 effector-immunity module of the Vibrio parahaemolyticus type VI secretion system.

The Journal of biological chemistry·2026

Same author

Prevention and management of nosocomial infections in patients undergoing extracorporeal membrane oxygenation: a summary of best evidence.

Frontiers in medicine·2026

Search research articles

Related Experiment Video

Updated: Jan 15, 2026

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Fast and robust drift correction for single-molecule localization microscopy.

Mengdi Hou^1,2, Jianyu Yang¹, Mingjie Yang¹

¹Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Institute of Applied Physics and School of Physics, Nankai University, Tianjin, China.

Nature Communications

|October 10, 2025

Summary

This summary is machine-generated.

Single-molecule localization microscopy (SMLM) drift correction is improved by NP-Cloud, a new method. This fast and robust technique enhances resolution and throughput for SMLM imaging.

More Related Videos

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Jan 15, 2026

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Biophysics
Optical Microscopy
Nanotechnology

Background:

Single-molecule localization microscopy (SMLM) requires precise correction of sample drifts during imaging.
Existing drift-correction methods for SMLM are often slow and unreliable, hindering resolution and throughput.

Purpose of the Study:

To introduce NP-Cloud, a novel, rapid, and robust method for correcting sample drifts in SMLM.
To demonstrate the effectiveness of NP-Cloud in improving SMLM data fidelity and speed.

Main Methods:

Developed NP-Cloud by pairing nearest molecules within SMLM data segments.
Calculated molecular displacements within a small search radius to reduce computational load.
Utilized continuously valued positions of super-localized molecules for accurate drift estimation.

Main Results:

NP-Cloud demonstrated superior robustness and fidelity in 3D drift correction using simulated and experimental SMLM data.
Achieved speed increases over 100-fold compared to single-referenced methods and over 10^4-fold over redundant cross-referenced approaches.
Provided excellent drift correction for diverse samples in seconds.

Conclusions:

NP-Cloud offers a significant advancement in SMLM drift correction.
The method provides a fast, robust, and practical solution, enhancing SMLM's resolution and throughput.
Facilitates high-performance SMLM imaging across various biological samples.