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Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography
Published on: August 12, 2025
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Deep learning-based high precision 3D ultrasound imaging for large size organ
Enxiang Shen1, Qiyue Zhou1, Caozhe Li1
1School of Electronic Science and Engineering, Nanjing University, Nanjing, China.
Medical & Biological Engineering & Computing
|October 11, 2025
Summary
Free-hand 3D ultrasound imaging uses deep learning but suffers cumulative errors. A new labeling strategy and pre-planned trajectories significantly improve accuracy and reduce distortion for better 3D imaging.
Area of Science:
- Medical Imaging
- Artificial Intelligence
- Biomedical Engineering
Background:
- Free-hand 3D ultrasound imaging offers advantages in device simplicity and user-friendliness.
- Deep learning networks are used for spatial coordinate prediction in this imaging modality.
- A key limitation is cumulative errors in spatial transformation prediction, leading to 3D image distortion, especially for large organs.
Purpose of the Study:
- To develop a novel labeling strategy to enhance deep learning network prediction accuracy in free-hand 3D ultrasound.
- To reduce cumulative errors in 3D ultrasound reconstruction.
- To improve the clinical applicability of 3D ultrasound imaging.
Main Methods:
- Proposed a labeling strategy based on the ultrasound image coordinate system.
- Implemented pre-planning of scanning trajectories to guide network prediction.
- Applied the method to spinal 3D ultrasound imaging in healthy volunteers and scoliosis patients.
Main Results:
- The proposed labeling strategy enhanced network prediction accuracy.
- Pre-planning trajectories significantly reduced cumulative error.
- Prediction accuracy improved by approximately 40%, and cumulative error was reduced by nearly 80% compared to existing methods.
Conclusions:
- The novel labeling strategy and trajectory guidance effectively reduce cumulative errors in free-hand 3D ultrasound.
- This approach shows significant potential for improving 3D reconstruction accuracy and facilitating wider clinical adoption of 3D ultrasound.
- The method is adaptable to various deep learning networks and tissue types.

