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Related Experiment Video

Updated: Jan 15, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

A Protocol Guide to Micro Milling for Bio-Microfluidics.

Hannah L Viola^1,2, Vishwa Vasani^1,3, Shuichi Takayama^1,4

¹The Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.

|October 13, 2025

Summary

This summary is machine-generated.

This study presents a practical protocol for micro milling, a computer numerical control (CNC) machining technique, to fabricate organs-on-a-chip. This method enhances rapid prototyping and the creation of complex 3D features for better physiological modeling.

Keywords:

CNC machining Micro mill Microfluidics Organ-on-a-chip PDMS casting Rapid prototyping

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Area of Science:

Biotechnology
Manufacturing Engineering
Medical Device Fabrication

Background:

Micro milling is a subtractive manufacturing technique for creating micro-scale 3D features from various substrates.
It offers advantages for rapid prototyping of biomicrofluidic devices and master molds compared to traditional methods.
Its application in organs-on-a-chip fabrication is limited due to the need for specialized computer numerical machining (CNC) expertise.

Purpose of the Study:

To provide practical guidelines for micro milling-based fabrication of organs-on-a-chip.
To address the knowledge gap in applying CNC techniques for organs-on-a-chip development.
To facilitate the design and prototyping of complex 3D features for improved physiological recapitulation.

Main Methods:

Detailed protocol for micro milling, including toolpath optimization.
Workflows using SolidWorks and Fusion software for model design and toolpath generation.
Stepwise guide covering model design, micro milling, device assembly, and cell culture.

Main Results:

Demonstration of micro milling for organs-on-a-chip fabrication.
Successful design and fabrication of master molds for a human airway-on-a-chip.
Validation of the fabricated molds in a recent publication.

Conclusions:

Micro milling can be effectively expanded for organs-on-a-chip fabrication.
This technique enhances the capacity for rapid device prototyping.
It enables the design of more complex 3D features that better mimic human physiology.