Related Experiment Video
Updated: Jan 15, 2026

A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis
Published on: March 9, 2015
Evaluation of Human Hair Absorption and Retention
Jeonghoon Yun1,2, Song Hee Kim1, Hyeyeon Yoo3
1Sunjin Clinical Research Center, Sunjin Beauty Science Co., Ltd., Seoul, Korea.
A new study shows hair care products significantly improve nutrient and moisture absorption into hair compared to water or no treatment. This 3D Raman spectroscopy method offers a better way to evaluate hair product efficacy and absorption.
Area of Science:
- Dermatology and Cosmetology
- Materials Science
- Spectroscopy
Background:
- Hair loss (alopecia) affects up to 50% of the population, impacting social and psychological well-being.
- Effective hair care relies on nutrient and moisture absorption, yet evaluation methods are limited.
- Understanding hair shaft penetration is key to developing effective hair loss treatments and products.
Purpose of the Study:
- To assess the absorption and retention of nutrients and moisture in human hair using 3D Raman spectroscopy.
- To compare the efficacy of a supplemental hair care product against untreated and water-treated hair.
- To establish a novel method for evaluating hair absorption and product effectiveness.
Main Methods:
- A double-arm study design was employed with three conditions: untreated hair, water-treated hair, and hair treated with a supplemental hair care product.
- 3D Raman spectroscopy with 3D imaging technology was utilized to analyze absorption amount, depth, and dryness at 30-minute intervals.
- Statistical analysis was performed to confirm the significance of observed differences in absorption and retention.
Main Results:
- Hair treated with the supplemental product demonstrated significantly higher absorption amounts and deeper penetration compared to untreated and water-treated hair (p < 0.05).
- Product-treated hair showed reduced dryness and maintained absorption parameters over 30 minutes (p < 0.05).
- 3D Raman visualization provided detailed spatial distribution and confirmed long-term retention of absorbed substances within hair fibers.
Conclusions:
- The study validates the superior absorption and retention capabilities of the tested hair care product in human hair.
- The developed 3D Raman spectroscopy method sets a new standard for evaluating hair absorption and product efficacy.
- This technique presents a promising tool for future clinical research and the development of advanced hair care solutions.
Related Concept Videos
Accessory Structures of the Skin: Hair Growth and Types
Accessory Structures of the Skin: Hair and Hair Follicles
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Hair Cells
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Measurement of Bioavailability: Pharmacokinetic Methods

