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Updated: Jul 31, 2026

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
Lysosomal pH Measurements via Dual-Imaging Microscopy
Emily Kirkham1, Sian Gardiner2, Emyr Lloyd-Evans1,2
1School of Biosciences, Cardiff University, Cardiff, UK.
Abstract:
Dual-wavelength ratiometric fluorescent probes are the current gold standard for generating a quantitative measure of subcellular compartment ion contents. This chapter describes the use of fluorescent probes to measure lysosomal pH, encompassing probe selection, calibration, instrumentation, and the miniaturization of assays to use for high-throughput readouts.
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