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This study presents a new method for isolating individual amino acids for radiocarbon dating. The technique ensures high accuracy for ultrasmall samples, improving measurement reliability in scientific research.

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Area of Science:

  • Analytical Chemistry
  • Geochemistry
  • Radiocarbon Dating

Background:

  • Compound-specific radiocarbon analysis requires pure amino acid samples.
  • Existing methods can be time-consuming and may not efficiently handle ultrasmall samples.

Purpose of the Study:

  • To develop an efficient method for isolating and purifying individual underivatized amino acids.
  • To enable reliable compound-specific radiocarbon analysis on ultrasmall samples.

Main Methods:

  • Utilized a semipreparative porous graphitic carbon column for single-step separation of proteinogenic amino acids.
  • Employed a mixed-mode ion-exchange column for further purification to minimize contamination.
  • Validated the method using standard amino acids for radiocarbon measurements.

Main Results:

  • Successfully isolated and purified all proteinogenic amino acids in a single chromatographic step.
  • Achieved F14C values closely aligned with original values for standard amino acids.
  • Determined a low blank contribution (1.0 ± 0.3 μg C) with high F14C accuracy (0.6 ± 0.12).

Conclusions:

  • The developed method is effective for isolating individual amino acids for compound-specific radiocarbon analysis.
  • This approach enhances measurement reliability, particularly for ultrasmall sample sizes.
  • The technique offers a robust solution for precise radiocarbon dating applications.