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Engineered pattern recognition receptors enhance broad-spectrum plant resistance.

Yuankun Yang1,2, Christina E Steidele3, Xianlong Huang4

  • 1Department of Plant Biochemistry, Center of Plant Molecular Biology (ZMBP), Eberhard-Karls-University of Tübingen, Tübingen, Germany. y.yang@biology.leidenuniv.nl.

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|October 14, 2025
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Summary

Plant pattern recognition receptors (PRRs) can be engineered for broad-spectrum crop resistance. Introducing Arabidopsis RLP23 into tomato enhances resistance to diverse pathogens without impacting yield.

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Area of Science:

  • Plant pathology
  • Genetics
  • Crop science

Background:

  • Intracellular immune receptors dominate conventional plant resistance breeding.
  • Cell-surface pattern recognition receptors (PRRs) are underexplored despite their potential for crop improvement.

Purpose of the Study:

  • To investigate the potential of PRRs for enhancing crop resistance.
  • To engineer the Arabidopsis receptor-like protein 23 (RLP23) for broad-spectrum disease resistance in crops.

Main Methods:

  • Heterologous expression of Arabidopsis RLP23 in tomato (Solanum lycopersicum).
  • Identification and engineering of the C-terminal domain of RLPs for enhanced efficacy.
  • Testing resistance against bacterial, fungal, and oomycete pathogens in engineered tomato plants.
  • Extension of the RLP engineering strategy to rice and poplar.

Main Results:

  • Arabidopsis RLP23 confers broad-spectrum resistance to pathogens from three kingdoms when introduced into tomato.
  • The C-terminal domain of RLPs is critical for compatibility and efficacy in heterologous expression.
  • Targeted engineering of the RLP23 C-terminal domain significantly enhances its disease resistance capabilities in tomato.
  • Engineered RLP23 provides robust resistance without compromising crop yield.
  • The RLP engineering strategy is applicable across different plant species like rice and poplar.

Conclusions:

  • PRR-based engineering offers a versatile framework for developing sustainable crop protection strategies.
  • RLP23 engineering provides a powerful tool for enhancing crop resilience against diverse pathogens.
  • This approach opens new avenues for improving global food security through advanced breeding techniques.