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Related Experiment Videos

Sensitive fluorescence assay for d,l-methadone.

C H Chi, B N Dixit

    Journal of Pharmaceutical Sciences
    |September 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    A new fluorescence assay allows for accurate quantification of d,l-methadone in biological samples. This method detects d,l-methadone in plasma and tissues, even with common interfering substances present.

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    Area of Science:

    • Analytical Chemistry
    • Forensic Toxicology

    Background:

    • Accurate quantification of d,l-methadone is crucial for clinical and forensic applications.
    • Existing analytical methods may face challenges with sensitivity or specificity in complex biological matrices.

    Purpose of the Study:

    • To develop and validate a sensitive fluorescence assay for the quantitative analysis of d,l-methadone.
    • To assess the assay's applicability in biological samples like plasma and tissue homogenates.

    Main Methods:

    • Extraction of d,l-methadone from deproteinized plasma or tissue homogenates at pH 9.2 into an organic solvent (25% isobutanol in ethylene dichloride).
    • Reaction of the extracted d,l-methadone with paraformaldehyde in concentrated sulfuric acid to form a fluorophore.
    • Quantification of fluorescence at 450 nm with excitation at 275 nm.

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    Main Results:

    • The developed fluorescence assay provides a reliable method for d,l-methadone quantification.
    • The assay demonstrates specificity, successfully measuring d,l-methadone in the presence of its metabolites and other common drugs.
    • Potential interferences were identified with amphetamine, meperidine, and quinine.

    Conclusions:

    • A robust fluorescence assay for d,l-methadone analysis in biological samples has been established.
    • This method offers a valuable tool for therapeutic drug monitoring and forensic toxicology.
    • Further validation may be required to mitigate interferences from specific compounds.