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Related Experiment Video

Updated: Jan 15, 2026

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

Design, fabrication, and characterization of a microbubble array microphysiological system.

Lukas B Jenkins¹, Hasibul Hasan Hredoy¹, Hossein Abolhassani¹

¹Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA.

|October 14, 2025

Summary

This summary is machine-generated.

This study introduces a novel microbubble-fluidic system for 3D tissue culture, improving nutrient delivery and preserving tissue fidelity under high flow rates for drug discovery.

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Area of Science:

Biotechnology
Tissue Engineering
Microfluidics

Background:

Microphysiological systems (MPS) are crucial for high-throughput 3D tissue culture and drug screening.
Microbubbles (MBs) offer a unique format for integration into MPS.
Current MPS face challenges in nutrient delivery, waste removal, and maintaining physiological relevance.

Purpose of the Study:

To develop and validate a hybrid microbubble-fluidic MPS for enhanced 3D tissue culture.
To investigate the impact of fluid dynamics on tissue culture within MBs.
To demonstrate the utility of this platform for drug discovery and toxicology.

Main Methods:

Computational fluid dynamics (CFD) simulations to model flow and solute diffusion within MBs.
Optical tracking of microspheres to validate CFD simulations.
Fabrication of a millifluidic MB device using 3D printing (FDM) and molding.
Culture of murine salivary gland tissues under dynamic flow conditions.

Main Results:

CFD simulations revealed significant velocity decoupling and shear dampening within MBs, allowing high channel flow rates while maintaining low-shear environments.
MBs with aspect ratios between 2 and 3 optimized nutrient transport and retention of cell-secreted factors.
3D printed MB devices maintained tissue fidelity, evidenced by preserved gene expression in cultured salivary gland tissues.
The hybrid system prevented tissue dislodgement under high flow and outperformed rectilinear wells in preventing lactate accumulation.

Conclusions:

The novel MB-fluidic MPS platform enables scalable, high-content 3D culture with improved physiological relevance.
This technology enhances nutrient/waste exchange and preserves tissue integrity, overcoming limitations of traditional microfluidic systems.
The platform is suitable for organoid, tumor spheroid, and tissue mimetic applications in drug discovery and toxicology.