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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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piRNA - Piwi-interacting RNAs02:57

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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siRNA - Small Interfering RNAs02:30

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Riboswitches01:56

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Precision RNAi in Tomato Using Synthetic Trans-Acting Small Interfering RNAs Derived From Minimal Precursors.

Ariel H Tomassi1, María Juárez-Molina1, Adriana E Cisneros1

  • 1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia, Spain.

Plant Biotechnology Journal
|October 14, 2025
PubMed
Summary

Researchers developed a novel RNA interference (RNAi) platform using minimal precursors for synthetic trans-acting small interfering RNAs (syn-tasiRNAs) in tomato. This breakthrough enables transgene-free gene silencing and enhances crop improvement strategies.

Keywords:
PVXRNAiTSWVantiviral resistancefunctional genomicsgene silencingsyn‐tasiRNAtomato

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Area of Science:

  • Plant molecular biology
  • Gene silencing mechanisms
  • Crop biotechnology

Background:

  • RNA interference (RNAi) is a key gene regulation process in plants.
  • Synthetic trans-acting small interfering RNAs (syn-tasiRNAs) offer precise gene silencing but face limitations in crop applications.
  • Existing methods require transgene integration and long precursor sequences.

Purpose of the Study:

  • To develop a novel syn-tasiRNA platform for Solanum lycopersicum (tomato).
  • To overcome limitations of current syn-tasiRNA applications in crops.
  • To establish efficient and versatile tools for precision RNAi in tomato.

Main Methods:

  • Designed minimal syn-tasiRNA precursors utilizing endogenous SlmiR482b microRNA targeting.
  • Constructed vectors for high-throughput cloning and expression of syn-tasiRNAs.
  • Utilized transgenic and transient virus-induced gene silencing (syn-tasiR-VIGS) systems.
  • Demonstrated transgene-free syn-tasiR-VIGS via crude extract delivery.

Main Results:

  • Minimal precursors efficiently produced functional syn-tasiRNAs in tomato.
  • Robust gene silencing of endogenous tomato genes was achieved.
  • Enhanced resistance against tomato spotted wilt virus was conferred.
  • Transgene-free syn-tasiR-VIGS demonstrated effective endogenous gene silencing.

Conclusions:

  • Minimal syn-tasiRNA precursors represent a versatile and efficient tool for precision RNAi in tomato.
  • The developed platform facilitates functional genomics and crop improvement.
  • This technology offers transgene-free gene silencing applications.