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Comprehensive overview: QuEChERS methods for mycotoxin determination in different matrices.

Ruifeng Lin1, Jiandan Yuan2, Huiqiang Feng3

  • 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
|October 14, 2025
PubMed
Summary
This summary is machine-generated.

Accurate mycotoxin detection in food is crucial for human health, but complex matrices and matrix effects hinder analysis. This review explores advanced QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methods for improved mycotoxin extraction and cleanup.

Keywords:
QuEChERSmatrix effectmycotoxinspre-treatment methods

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Area of Science:

  • Food Safety and Analytical Chemistry
  • Environmental Science and Toxicology

Background:

  • Mycotoxin analysis in food is vital for public health, yet faces challenges like matrix effects (MEs) and complex sample matrices.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS), while sensitive, is significantly limited by MEs, necessitating robust sample preparation.
  • The Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method offers a green, efficient approach for mycotoxin detection in food.

Purpose of the Study:

  • To comprehensively review recent advancements in modified QuEChERS methods for multi-mycotoxin analysis in food matrices.
  • To evaluate extraction and cleanup techniques, including salting-out methods and sorbent materials, for overcoming analytical challenges.
  • To provide future perspectives for mycotoxin analysis based on improvements in QuEChERS methodologies.

Main Methods:

  • Systematic literature search of Web of Science, Scopus, and PubMed (2015-2025) using keywords like 'QuEChERS', 'mycotoxin', 'extraction', and 'cleanup'.
  • Inclusion criteria focused on validated methods and real sample analysis.
  • Review of traditional sorbents and advanced nanomaterials in modified QuEChERS approaches.

Main Results:

  • Modified QuEChERS methods show promise in addressing matrix effects and improving multi-mycotoxin detection sensitivity and selectivity.
  • Diverse extraction and cleanup strategies, including salting-out and novel sorbents, demonstrate varying efficiencies in removing interfering substances.
  • The review systematically analyzes extraction techniques and predicts their removal efficiency for different interfering substances.

Conclusions:

  • Modified QuEChERS methods are essential for accurate multi-mycotoxin analysis in complex food matrices.
  • Continued development of sorbent materials and extraction techniques is crucial for enhancing analytical performance.
  • Future research should leverage QuEChERS advancements for improved food safety monitoring and regulatory compliance.