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Updated: Jul 18, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
Commentary: a review of technical considerations for planning an RNA-Sequencing experiment
Rashi Verma1,2, Amanda Savaria-Butler3, Francisco J Enguita4
1Morehouse School of Medicine, Institute of Translational Genomic Medicine, Atlanta, GA, 30310, USA.
Abstract:
There are a bewildering number of variables to consider when planning an RNA sequencing study (RNA-Seq). Here we discuss some of the considerations for an investigator initiating such studies, with a focus on library preparation, depletion techniques, considerations for data analysis, and finally, we consider the cost of such a study. The goal of this summary is to make the researcher aware of such considerations to be able to plan an analysis strategy prior to initiating an RNA-Seq study.
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