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Published on: December 17, 2016
[Assembly of a Complex of an Immature 30S Ribosomal Subunit and the Era GTPase Maturation Factor from Staphylococcus
E A Klochkova1,2, A D Biktimirov1, A G Bikmullin1
1Kazan Federal University, Kazan, 420008 Russia.
Abstract:
Era GTPase of Staphylococcus aureus is a ribosome maturation factor. Era GTPase is involved in the late stages of small (30S) ribosome subunit assembly and regulates formation of the entire 70S ribosome. Study of the structure of the Era and 30S subunit complex from S. aureus will help in studying the processes of ribosome maturation and the mechanisms of protein synthesis regulation in this pathogenic microorganism. This paper presents protocols for obtaining Era GTPase and immature 30S ribosome subunits from S. aureus, as well as a protocol for assembling their complex for further structural studies.
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