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Recently, the development of olefin metathesis polymerization advanced the field of polymer synthesis. Simply put, the reorganization of substituents on their double bonds between two olefins in the presence of a catalyst is known as the olefin metathesis reaction. The use of metathesis reaction for polymer synthesis is called olefin metathesis polymerization.
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Programmable Artificial-Cellular Membrane Dynamics via Ring-Closing Metathesis.

Rei Hamaguchi1, Damian Alexander Graf2, Kazushi Kinbara1,3

  • 1School of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.

Journal of the American Chemical Society
|October 15, 2025
PubMed
Summary
This summary is machine-generated.

Researchers dynamically controlled lipid membrane phase separation using catalysis. A biotin-streptavidin artificial metalloenzyme triggered ring-closing olefin metathesis, releasing fatty acids to dissolve membrane domains.

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Materials Science

Background:

  • Cellular membranes feature lateral phase-separated structures crucial for biological functions.
  • Controlling these domains could lead to smart vesicles with life-like behaviors.

Purpose of the Study:

  • To demonstrate dynamic control over lipid membrane lateral phase separation using catalysis.
  • To engineer artificial metalloenzymes for membrane-associated reactions.

Main Methods:

  • Utilized ring-closing olefin metathesis (RCM) catalyzed by a biotin-streptavidin artificial metalloenzyme (ArM) on lipid membrane surfaces.
  • Designed a substrate that releases decanoic acid upon RCM, integrating into the lipid bilayer.
  • Genetically optimized the ArM for enhanced catalytic activity.

Main Results:

  • Achieved the first example of catalytic control over lateral phase separation in lipid membranes.
  • Observed the disappearance of lipid domains due to decanoic acid incorporation.
  • Genetic optimization of the ArM increased catalytic activity threefold, promoting larger lipid domain budding.

Conclusions:

  • Catalysis offers a novel strategy for dynamic control of membrane phase separation.
  • Artificial metalloenzymes can be engineered for precise spatiotemporal control of membrane properties.
  • This work paves the way for creating responsive biomimetic materials.