Related Experiment Video
Updated: Jan 15, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
[Distribution Characteristics of Antibiotic Resistance Genes in Soils Under Different Land Use Patterns in Beijing]
Xiang Zhao1, Jian Wang2,3, Jun-Xiang Xu1
1Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Abstract:
To reveal the distribution characteristics of antibiotic resistance genes (ARGs) in soil under different land use patterns in Beijing, 75 soil samples, from agricultural field, vegetable field, greenhouse vegetable field, and orchard, were selected and analyzed by high-throughput qPCR. The results showed that a total of 195 ARGs and 9 MGEs were detected in all samples and that the top three classes of detected ARGs were β lactam, multidrug, and aminoglycoside resistance genes. The highest number of ARGs was detected in greenhouse vegetable field samples, and the lowest number of ARGs was detected in agricultural field samples. Among the different regions, higher relative abundances of ARGs occurred in greenhouse vegetable field soil in the districts of Pinggu, Changping, Tongzhou, and Haidian; whereas higher abundances in agricultural field, vegetable field, and orchard occurred in Yanqing. The lowest relative abundance of ARGs was detected in Fangshan. In greenhouse vegetable field, there was a significant negative correlation between available potassium (AK) and aminoglycoside and β lactam resistance genes (P<0.05); in orchard, available phosphorus (AP) showed a significant positive correlation with bacterial number (P<0.01).
Related Concept Videos
Antibiotic Selection
Development of Antibiotic Resistance
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Transformation

