Inhibition of Pyruvate Dehydrogenase Kinase 2 Alleviates Concanavalin A-Induced Acute Liver Injury by Regulating...

Pyruvate dehydrogenase kinase 2 (PDK2) regulates glucose metabolism; however, its role in liver injury development is unknown. Therefore, this study aimed to explore the effects of PDK2 on liver injury using a concanavalin A (Con A)-induced acute liver injury mouse model. To understand the mechanisms by which PDK2 affects liver injury pathogenesis, network pharmacology analysis was performed to pinpoint the potential target genes and signaling pathways involved. Con A was used to induce acute liver injury in mice. Thereafter, PDK2 differential expression levels in the liver tissue of normal and liver injury mice were analyzed, and the effects of PDK2 inhibition on Con A-induced liver damage were assessed through biochemical analysis and observation of hepatic histopathological changes. Enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, flow cytometry, and western blotting were used to explore the possible mechanisms by which PDK2 affects liver injury. PDK2 was involved in liver injury progression, and pharmacological inhibition of PDK2 notably increased mouse survival, decreased serum alanine transaminase and aspartate aminotransferase levels, attenuated hepatic histopathological damage and apoptosis, and inhibited pro-inflammatory cytokine secretion and the activation of the mitogen-activated protein kinase/nuclear factor kappa B (MAPK/NF-κB) signaling pathway. Pharmacological inhibition of PDK2 markedly alleviated hepatic oxidative stress and elevated myeloid-derived suppressor cells (MDSCs) in the livers of acute liver injury mice. Overall, inhibiting PDK2 ameliorates Con A-induced acute liver injury by enhancing MDSCs accumulation and suppressing oxidative stress, the MAPK/NF-κB signaling pathway, and inflammation. These findings identify PDK2 as a promising therapeutic target for immune-mediated hepatitis.