Related Experiment Video
Updated: May 2, 2026

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Published on: May 1, 2012
Monitoring Single DNA Docking Site Activity with Sequential Modes of an Optoplasmonic Whispering-Gallery Mode
Narima Eerqing1,2, Ekaterina Zossimova1,3, Sivaraman Subramanian1
1Living Systems Institute, University of Exeter, Exeter EX4 4QD, UK.
Abstract:
In recent years, there has been rapid advancement in single-molecule techniques, driven by their unparalleled precision in studying molecules whose sizes are beyond the diffraction limit. Among these techniques, optoplasmonic whispering gallery mode sensing has demonstrated great potential in label-free single-molecule characterization. It combines the principles of localized surface plasmon resonance (LSPR) and whispering gallery mode (WGM) sensing, offering exceptional sensing capabilities, even at the level of single ions. However, current optoplasmonic WGM sensing operates in a multiplexed channel, making it challenging to focus on individual binding sites of analyte molecules. In this article, we characterize different binding sites of DNA analyte molecules hybridizing to docking strands on the optoplasmonic WGM sensor, using the ratio of the resonance shift between sequential polar WGM modes. We identify specific docking sites that undergo transient interactions and eventually hybridize with the complementary analyte strands permanently.
More Related Videos
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays
Sanger Sequencing
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Modern Molecular Taxonomy
Microbial Biosensors

