Related Experiment Video
Updated: May 13, 2026
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
High Salivary 3-Nitrotyrosine Levels in Periodontitis
Leonardo Lorente1, Esther Hernández Marrero2, Pedro Abreu González3
1Intensive Care Unit, Hospital Universitario de Canarias, Ofra s/n, 38320 San Cristóbal de La Laguna, Tenerife, Spain.
Saliva analysis reveals higher 3-nitrotyrosine (3-NT) levels in periodontitis patients, suggesting a link between this oxidative stress marker and the disease. Elevated salivary 3-NT may aid in diagnosing periodontitis.
Area of Science:
- Biochemistry
- Periodontology
- Biomarkers
Background:
- Tyrosine, an amino acid, oxidizes to 3-nitrotyrosine (3-NT) upon exposure to reactive species.
- Elevated 3-NT is found in periodontal tissues of periodontitis patients.
- Previous studies suggest a correlation between 3-NT and periodontitis severity.
Purpose of the Study:
- Compare salivary 3-NT concentrations in periodontitis patients and healthy controls.
- Determine the association between salivary 3-NT and periodontitis.
- Evaluate salivary 3-NT as a diagnostic marker for periodontitis.
Main Methods:
- Prospective study measuring salivary 3-NT in 60 periodontitis patients and 66 controls.
- Multivariate logistic regression identified independent predictors of periodontitis.
- Receiver operating characteristic (ROC) analysis assessed diagnostic accuracy.
Main Results:
- Salivary 3-NT > 4.25 ng/mL independently associated with periodontitis (OR=3.22, p=0.02).
- Age and non-smoking status were also independently linked to periodontitis.
- ROC analysis showed salivary 3-NT had 62% AUC for periodontitis diagnosis (p=0.02).
Conclusions:
- Salivary 3-NT concentrations are significantly higher in individuals with periodontitis.
- A positive association exists between elevated salivary 3-NT and periodontitis.
- Salivary 3-NT shows potential as a diagnostic biomarker for periodontitis.
More Related Videos
07:32Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
07:15Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
