Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Acute Kidney Injury I: Introduction01:22

Acute Kidney Injury I: Introduction

564
Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
564
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

876
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
876
Acute Pancreatitis I: Introduction01:27

Acute Pancreatitis I: Introduction

1.1K
Pancreatitis is inflammation of the pancreas, an organ located behind the stomach. It can be either acute or chronic.
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
1.1K
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

257
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
257

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Case Report: Allogeneic hematopoietic stem cell transplantation in a patient with acute myeloid leukemia and hepatitis E.

Frontiers in oncology·2026
Same author

Vancomycin Exposure Dynamics and Clinical Outcomes in Critically Ill Patients: A Retrospective Cohort Study.

Antibiotics (Basel, Switzerland)·2026
Same author

Systematic assessment for potential endogenous normalizers for microRNA analysis in fecal samples.

Scientific reports·2026
Same author

SARS-CoV-2 infection, viral burden, and variant-specific outcomes in hospitalized patients with heart failure.

European journal of internal medicine·2026
Same author

Targeting SMYD2 improves immunotherapy response in experimental hepatocellular carcinoma.

Molecular therapy. Oncology·2026
Same author

Are CD4<sup>+</sup> T-Cell Counts Associated with <i>Pneumocystis jirovecii</i> Detection in Hospitalized Patients with Liver Disease? A Retrospective Exploratory Pilot Analysis.

Livers·2026

Related Experiment Video

Updated: Jan 15, 2026

Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen
09:44

Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen

Published on: November 27, 2019

11.0K

RAC1 as a novel therapeutic target for acute liver failure.

Barbara Bueloni1,2,3, Esteban Fiore1,2,4, María José Cantero1,2,4

  • 1Hepatology and Gene Therapy Program, Instituto de Investigaciones en Medicina Traslacional, CONICET - Universidad Austral, Av. Pte. Perón 1500, B1629AHJ, Pilar, Buenos Aires, Argentina.

JHEP Reports : Innovation in Hepatology
|October 16, 2025
PubMed
Summary

Targeting RAC1 (Rho GTPase) protein with 1D-142 effectively reduces liver injury, inflammation, and oxidative stress in acute liver failure (ALF) models. This demonstrates RAC1 inhibition as a promising therapeutic strategy for ALF.

Keywords:
Autoimmune hepatitisDrug induced liver injuryHBV associated liver failureHepatocyte apoptosisMacrophage polarizationMolecular inhibitorsRHO GTPases

More Related Videos

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride CCl4 Exposure Through an Orogastric Tube
06:12

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride CCl4 Exposure Through an Orogastric Tube

Published on: April 28, 2020

11.9K
Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
11:24

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Published on: February 10, 2018

14.7K

Related Experiment Videos

Last Updated: Jan 15, 2026

Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen
09:44

Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen

Published on: November 27, 2019

11.0K
Inducing Acute Liver Injury in Rats via Carbon Tetrachloride CCl4 Exposure Through an Orogastric Tube
06:12

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride CCl4 Exposure Through an Orogastric Tube

Published on: April 28, 2020

11.9K
Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
11:24

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Published on: February 10, 2018

14.7K

Area of Science:

  • Hepatology
  • Molecular Biology
  • Immunology

Background:

  • Acute liver failure (ALF) involves severe inflammation and oxidative stress with limited treatments.
  • The Rho GTPase RAC1 plays a key role in regulating these pathological processes in ALF.

Purpose of the Study:

  • To investigate the therapeutic potential of inhibiting RAC1 in ALF.
  • To evaluate the efficacy of the RAC1 inhibitor 1D-142 in preclinical ALF models and human liver explants.

Main Methods:

  • Transcriptomic analysis of human ALF datasets (GSE38941, GSE80751) and mouse livers.
  • Induction of ALF in mice using concanavalin A, acetaminophen, or D-galactosamine/lipopolysaccharide.
  • In vivo and in vitro administration of the RAC1 inhibitor 1D-142 to mice, hepatocytes, and macrophages.
  • Treatment of human ALF liver explants with 1D-142.

Main Results:

  • RAC1 was identified as an upstream regulator correlating with immune activation and oxidative stress in human ALF.
  • 1D-142 administration significantly ameliorated liver injury in murine ALF models at various stages.
  • RAC1 inhibition reduced reactive oxygen species, inflammatory cell migration, cytokine production, and hepatocyte death.
  • 1D-142 treatment decreased necrosis and pro-inflammatory gene expression in human liver explants.

Conclusions:

  • RAC1 drives sterile inflammation and oxidative stress in ALF.
  • Pharmacological inhibition of RAC1 with 1D-142 demonstrates protective effects against liver injury in preclinical and human models.
  • RAC1 represents a potential therapeutic target for ALF, warranting further clinical investigation.