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Regulating Promiscuous Catalysis via Substrate-Induced Transient Assembly.

Ayan Chatterjee1, Maximilian Schuler1,2, Marius G Braun1

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|October 16, 2025
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Summary
This summary is machine-generated.

Researchers created a transient nanostructure from peptides and Fmoc-glycine that mimics primitive enzymes. This structure catalyzes multiple reactions, offering insights into early life chemistry and the origin of catalysis.

Keywords:
Carbamate cleavageEmergent catalysisNonequilibrium assemblyPeptidesPromiscuity

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Area of Science:

  • * Origin of life studies
  • * Systems chemistry
  • * Supramolecular chemistry

Background:

  • * Substrate-induced assembly is crucial for enzyme-driven reactions in nature.
  • * Synthetic substrate analogues enhance peptide fold catalysis, mimicking primitive enzymes.
  • * The role of catalytic promiscuity in prebiotic chemistry diversification is understudied.

Purpose of the Study:

  • * To investigate substrate-induced co-assembly for primitive catalysis.
  • * To explore catalytic promiscuity in a synthetic system.
  • * To understand the emergence of broad substrate scope in early life.

Main Methods:

  • * Formation of a transient co-assembly between a lysine-rich peptide and Fmoc-glycine under nonequilibrium conditions.
  • * Characterization of the nanostructure's microenvironment.
  • * Observation of orthogonal hydrolysis and C═N condensation reactions.
  • * Analysis of Fmoc group cleavage and its effect on assembly stability.

Main Results:

  • * A novel transient, substrate-induced co-assembly was formed.
  • * The nanostructure facilitated orthogonal hydrolysis and C═N condensation reactions.
  • * Carbamate bond cleavage of the Fmoc group led to gradual destabilization and collapse of the co-assembly.
  • * Catalytic promiscuity was encoded into assembling building blocks under kinetic control.

Conclusions:

  • * The study demonstrates a synthetic system exhibiting catalytic promiscuity, mimicking primitive enzymes.
  • * This co-assembly provides a suitable microenvironment for multiple chemical transformations.
  • * Findings shed light on the emergence of primitive catalysis with broad substrate scope at the origin of life.