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Related Experiment Video

Updated: Jan 15, 2026

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Exploring bacteria-surface interactions with a fluorescent membrane tension probe.

M Carmen Gonzalez-Garcia¹, Daniel Ballesteros², Jaime J Hernández¹

¹Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia), Madrid 28049, Spain.

Proceedings of the National Academy of Sciences of the United States of America

|October 16, 2025

Summary

This summary is machine-generated.

This study uses fluorescence lifetime imaging microscopy (FLIM) with Flipper-TR to measure bacterial membrane tension during surface interactions. The method reveals how bacterial adhesion and material properties affect membrane mechanics.

Keywords:

bacteria cell–material interface fluorescence lifetime imaging membrane tension probe nanotopographies

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Area of Science:

Microbiology
Biophysics
Materials Science

Background:

Bacterial surface interactions are crucial for biofilm formation, biomaterials, and biosensing.
Understanding the biophysical mechanisms at the biointerface requires advanced microscopy.
Current methods for studying bacterial membrane tension at surfaces are limited.

Purpose of the Study:

To investigate bacterial membrane tension during surface interactions using a novel fluorescence microscopy approach.
To assess the utility of the tension reporter Flipper-TR for studying bacterial biophysics.
To explore how different surfaces and bacterial adhesion mechanisms influence membrane tension.

Main Methods:

Employing fluorescence lifetime imaging microscopy (FLIM) with the tension reporter Flipper-TR.
Staining both Gram-positive and Gram-negative bacterial membranes with Flipper-TR.
Analyzing fluorescence lifetime variations to infer membrane tension differences.

Main Results:

Flipper-TR successfully stained bacterial membranes, showing shorter fluorescence lifetimes than in eukaryotic cells.
Membrane tension varied spatially within bacterial cells, correlating with cell wall architecture.
Flipper-TR detected distinct membrane tension changes based on surface coatings and adhesion mechanisms.
Changes in membrane tension were observed upon exposure to nanostructured substrates.

Conclusions:

Flipper-TR is a valuable tool for studying bacterial membrane tension and its response to surface interactions.
This technique provides insights into the mechanical aspects of bacterial-material interfaces.
Findings can inform the design of materials to control bacterial behavior for applications like biofilm prevention.