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Related Experiment Video

Updated: Jan 14, 2026

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
08:40

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Published on: March 21, 2017

8.0K

Elecsys CSF AD immunoassays: Sample stability for a new pre-analytical protocol for fresh CSF.

Ekaterina Manuilova1, Isabelle Schrurs2, Sandra Rutz1

  • 1Roche Diagnostics GmbH, Penzberg, Germany.

Alzheimer'S & Dementia : the Journal of the Alzheimer'S Association
|October 17, 2025
PubMed
Summary

Cerebrospinal fluid biomarkers for Alzheimer's disease diagnosis are stable under specific storage conditions. Optimal storage for Elecsys immunoassays includes refrigeration for up to 15 days or freezing for up to 8 weeks.

Keywords:
Alzheimer's diseaseAβ42Elecsys CSF immunoassaybiomarkersfresh CSFpre‐analytical handlingp‐tausample stabilitystorage conditionst‐tau

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Area of Science:

  • Biochemistry
  • Neuroscience
  • Clinical Diagnostics

Background:

  • Cerebrospinal fluid (CSF) biomarkers, including amyloid beta 1-42 (Aβ42), phosphorylated tau (p-tau181), and total tau (t-tau), are crucial for diagnosing Alzheimer's disease.
  • Accurate quantification of these biomarkers is essential for reliable diagnostic performance.

Purpose of the Study:

  • To evaluate the stability of Aβ42, p-tau181, and t-tau in human CSF samples under various storage conditions.
  • To determine optimal storage parameters for maintaining biomarker integrity for Elecsys CSF immunoassays.

Main Methods:

  • Prospective analysis of fresh CSF samples.
  • Assessment of biomarker stability based on storage time, temperature (15-25°C, 2-8°C), and freeze/thaw cycles.
  • Utilized Elecsys® CSF immunoassays for biomarker quantification.

Main Results:

  • Aβ42, p-tau181, and t-tau remained stable at 2-8°C for up to 15 days and at -25°C to -15°C for up to 8 weeks.
  • p-Tau181 and t-tau showed stability at 15-25°C for up to 8 days and at -25°C to -15°C for 12-15 weeks.
  • Aβ42 showed slight recovery decline after storage at 15-25°C for ≤8 days and -25°C to -15°C for 12-15 weeks, and after one freeze/thaw cycle.

Conclusions:

  • Recommended optimal storage for CSF samples for Elecsys immunoassays: 15-25°C for ≤5 days, 2-8°C for ≤15 days, and -25°C to -15°C for ≤8 weeks.
  • Adherence to these storage guidelines ensures reliable Alzheimer's disease biomarker measurements.