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Related Concept Videos

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
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Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
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Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
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Advanced Large DNA Manipulation Technologies for Constructing Microbial Cell Factories.

Zubin Pan1,2,3,4, Yi Wu1,2,3

  • 1State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, China.

ACS Synthetic Biology
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

Large DNA manipulation technologies are advancing microbial cell factories for biomanufacturing. These tools improve synthetic biology by enabling efficient construction of complex genetic systems for higher product yields.

Keywords:
large DNA assemblylarge DNA cloninglarge DNA deliverylarge DNA rearrangementmicrobial cell factoriessynthetic biology

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Area of Science:

  • Synthetic Biology
  • Biotechnology
  • Metabolic Engineering

Background:

  • Microbial cell factories are crucial for biomanufacturing.
  • Improving their efficiency and product yield is key for synthetic biology and industrial applications.
  • Large DNA manipulation technologies have rapidly advanced, supporting DNA cloning, assembly, delivery, and rearrangement.

Purpose of the Study:

  • To systematically review core principles and recent advances in large DNA manipulation techniques.
  • To highlight the role of these techniques in constructing complex biosynthetic pathways and optimizing metabolic networks.
  • To explore the emerging trends and applications of large DNA manipulation in advancing microbial cell factories.

Main Methods:

  • Review of four categories of large DNA manipulation techniques.
  • Analysis of their application in accessing complex gene clusters.
  • Examination of their use in constructing multigene pathways and introducing genetic modules into microbial chassis.

Main Results:

  • Large DNA manipulation techniques are vital for accessing complex biosynthetic gene clusters.
  • These methods facilitate the construction of multigene pathways and the introduction of genetic modules into microbial chassis.
  • Applications include structural rewiring and modular reconstruction for metabolic network optimization.

Conclusions:

  • Large DNA manipulation technologies are essential for advancing microbial cell factory construction.
  • These techniques offer powerful tools for synthetic biology and industrial biomanufacturing.
  • Continued development and application of these technologies will lead to high-performance microbial cell factories.