Use of mouse knockout models to validate the specificity of monoclonal antibodies

The use of antibodies as research tools has contributed to the characterization of specific biomolecules and the understanding of their role in biological processes and disease. However, the usefulness of antibodies is only enhanced by their specificity for the target molecule because off-target and non-specific reactivity confounds results. Using accessible websites and standard hybridoma technology, we describe the development of targeted antibodies with high specificity and antibody validation using genetically deficient animals.