Rutin and skeletal muscle ischemia-reperfusion injury: Effects of acute treatment

Because skeletal muscle ischemiareperfusion injury (IRI) is mediated primarily by oxidative stress and leukocyte infiltration, the effect of rutin was evaluated in an experimental model.

An IRI model (4 +2 h) was established by the application of a rubber band around the groin of the rats. The sham control group (SC) underwent the same procedures except for the application of the elastic band. Rutin (10, 50, and 100 mg/kg/i.p.) was administered 30 min before the reperfusion phase. Serum creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) and muscle total oxidant status (TOS), total antioxidant status (TAS), myeloperoxidase (MPO), E-selectin, P-selectin, L-selectin, intercellular adhesion molecule-1 (ICAM-1), interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha (TNF-α) levels were measured.

Compared to the SC group, the IRI group exhibited significantly elevated levels of CPK, LDH, ICAM-1, E-selectin, L-selectin, IL-1β, IL-6, and TNF-α. Following the administration of rutin (100 mg/kg), CPK, LDH, IL-1β, and IL-6 levels were not significantly lower, while ICAM-1, E-selectin, L-selectin, and TNF-α levels were significantly reduced. Although no significant differences in the TOS and MPO values were detected between the groups, there were increasing and decreasing trends in the IRI- and rutin-administered groups, respectively. The decreased level of TAS observed in the IRI group compared with the SC group was significantly prevented by 100 mg/kg rutin.

Rutin may have a favorable effect on IRI, and some of the markers related to neutrophil infiltration may play a key role in this phenomenon.