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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
Published on: June 20, 2018
Metabolic heavyweight: thick ascending limb or proximal tubule? Next round
Kevin A Hurtado1, Leslie S Gewin2
1Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA.
Abstract:
In this issue of Kidney International, investigators define the thick ascending limb as the most metabolically active cell in the kidney using a combination of single-cell techniques and mass spectrometry imaging with stable isotope tracing studies. They show that the thick ascending limb is particularly susceptible to acute kidney injury and resistant to repair. These data are discussed in the context of the longstanding debate regarding the relative roles of thick ascending limb and proximal tubules in acute kidney injury.
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