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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Updated: Jan 14, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
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DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.

Hon Wing Liu1, Stephan Gruber2

  • 1Department of Fundamental Microbiology (DMF), Faculty of Biology and Medicine (FBM), University of Lausanne (UNIL), Lausanne, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a stable DNA-Dynabeads bioconjugate using SNAP-tag technology, overcoming dissociation risks of non-covalent methods for biochemistry and nanobiology applications.

Keywords:
DNA roadblocksDynabeadsJetABCDSMC complexesSNAP-tagWadjet

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Area of Science:

  • Biochemistry
  • Nanobiology
  • Molecular Biology

Background:

  • Polymer beads like Dynabeads are crucial for DNA and protein isolation in biochemistry and nanobiology.
  • Existing DNA-bead linkages use non-covalent methods (e.g., biotin-streptavidin), risking dissociation and impacting data integrity.

Purpose of the Study:

  • To develop a robust protocol for stable covalent DNA-Dynabeads bioconjugation.
  • To eliminate spontaneous dissociation issues associated with non-covalent DNA-bead attachments.

Main Methods:

  • Utilized SNAP-tag technology for covalent DNA-Dynabeads anchoring.
  • Generated benzylguanylated (BG) closed covalent circular DNA (BG-cccDNA).
  • Described conjugation steps, enzymatic treatment, and DNA elution.

Main Results:

  • Successfully created stable covalent bioconjugates between DNA and Dynabeads.
  • Demonstrated the protocol's efficacy in a study of DNA roadblocks on bacterial SMC motor activity.

Conclusions:

  • The SNAP-tag protocol provides a reliable method for DNA-Dynabeads conjugation, enhancing data accuracy in biochemical and nanobiological studies.
  • This technique enables new investigations into DNA-protein interactions and molecular motor functions.